Cloned (Comment) | Organism |
---|---|
recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)pLysS | Shewanella woodyi |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
23000 | - |
2 * 74500 + 2 * 23000, about, sedimentation equilibrium analysis, the dual-functioning diguanylate cyclase/phosphodiesterase enzyme, formed by heme-nitric oxide/oxygen binding protein and cyclic-di-GMP processing enzyme, builds a tetrameric structure | Shewanella woodyi |
74500 | - |
2 * 74500 + 2 * 23000, about, sedimentation equilibrium analysis, the dual-functioning diguanylate cyclase/phosphodiesterase enzyme, formed by heme-nitric oxide/oxygen binding protein and cyclic-di-GMP processing enzyme, builds a tetrameric structure | Shewanella woodyi |
74500 | - |
2 * 74500, in solution, sedimentation equilibrium analysis | Shewanella woodyi |
144300 | - |
dimeric enzyme, sedimentation equilibrium analysis | Shewanella woodyi |
197200 | - |
tetrameric heterotetramer of dual-functioning diguanylate cyclase/phosphodiesterase enzyme complex, sedimentation equilibrium analysis | Shewanella woodyi |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Shewanella woodyi | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3)pLysS cell-free supernatant by nickel affinity chromatography | Shewanella woodyi |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 74500, in solution, sedimentation equilibrium analysis | Shewanella woodyi |
More | wild-type heme-nitric oxide/oxygen binding protein , SwH-NOX, alone exists as a monomer and the cyclic-di-GMP phosphodiesterase, SwHaCE, as a dimer | Shewanella woodyi |
tetramer | 2 * 74500 + 2 * 23000, about, sedimentation equilibrium analysis, the dual-functioning diguanylate cyclase/phosphodiesterase enzyme, formed by heme-nitric oxide/oxygen binding protein and cyclic-di-GMP processing enzyme, builds a tetrameric structure | Shewanella woodyi |
Synonyms | Comment | Organism |
---|---|---|
diguanylate cyclase/phosphodiesterase | - |
Shewanella woodyi |
H-NOX-associated cyclic-di-GMP processing enzyme | - |
Shewanella woodyi |
HaCE | - |
Shewanella woodyi |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Shewanella woodyi |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Shewanella woodyi |
General Information | Comment | Organism |
---|---|---|
additional information | an heme-nitric oxide/oxygen binding protein, H-NOX , in the biofilm-dwelling bacterium Shewanella woodyi mediates NO-induced biofilm dispersal. SwH-NOX is cocistronic with a gene encoding a dual-functioning diguanylate cyclase/phosphodiesterase enzyme, designated here as H-NOX-associated cyclic-di-GMP processing enzyme, HaCE. Interaction analysis of recombinant enzyme with recombinnat wild-type SwH-NOX and mutants E16K, F17A, and E20K, the mutants show a weaker binding of the enzyme HaCE compared to the wild-type, overview | Shewanella woodyi |
physiological function | the dual-functioning diguanylate cyclase/phosphodiesterase enzyme is responsible for regulating the intracellular concentrations of cyclic-di-GMP, a secondary signaling molecule essential to biofilm formation in bacteria | Shewanella woodyi |